We're excited to spotlight a groundbreaking study that leveraged cutting-edge 10x Genomics technology to chart the cellular and gene expression landscape of the developing human heart at single-cell resolution.
Scientific Context: The study aimed to unravel how gene expression and cell types are organized during early human heart development. By combining spatial transcriptomics, scRNA-seq (Chromium), and in situ sequencing, the team mapped cell-type-specific gene expression to precise anatomical regions across three developmental stages.
How were 10x Genomics' products used?
The research team used the 10x Genomics Chromium platform for single-cell RNA sequencing (scRNA-seq), generating over 3,700 single-cell profiles from embryonic heart tissue. This enabled the identification of diverse cardiac cell types—including multiple cardiomyocyte populations, fibroblasts, endothelial cells, neural crest cells, and Schwann progenitor cells—at a crucial developmental stage. The single-cell data were integrated with spatial transcriptomics and in situ sequencing to create a comprehensive, organ-wide 3D atlas of gene expression and cell distribution.
Impact: 10x Genomics Chromium technology was essential for dissecting cellular heterogeneity and providing the single-cell resolution needed to build a detailed heart atlas. The integration of these data revealed new cell populations, clarified spatial relationships, and produced a publicly available web resource for the scientific community. This work sets a new standard for organ-wide developmental mapping and provides a template for similar studies in other tissues.
BonsaiLab: Your 10x Genomics Partner in Spain and Portugal
As the official distributor of 10x Genomics in Spain and Portugal, BonsaiLab is here to help you bring these transformative technologies to your research, our team is ready to support you.
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Curious to learn more?
Read the full study: Here